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Epicypher欣博盛生物—CUTANA™ E. coli Spike-in DNA

更新時間：2023-11-08   點擊次數(shù)：312次

來源于Escherichia coli（E.coli）的片段DNA可以用作核酸酶靶向切割和釋放（CUT&RUN）的實驗標準化的spike-in對照。產(chǎn)品CUTANA™ E. coli Spike-in DNA含有足夠的材料，可用于100-200個CUT&RUN樣本（高豐度和低豐度靶標的范圍）。


產(chǎn)品詳情

保存溫度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

運輸溫度: Room temp. Can go on frozen cold packs or dry ice.

產(chǎn)品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.


驗證數(shù)據(jù)

Epicypher熱銷產(chǎn)品——CUTANA™ E. coli Spike-in DNA

Figure 1: DNA gel data

E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E. 

coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in 

DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions 

of DNA molecular weight markers are indicated.


Epicypher熱銷產(chǎn)品——CUTANA™ E. coli Spike-in DNA

Figure 2: CUT&RUN sequencing data

CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched 

for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher 

13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads 

aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE: 

Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 - 

5%) of total sequencing reads.


訂購詳情

貨號

產(chǎn)品名稱

規(guī)格

18-1401

CUTANA™ E. coli Spike-in DNA

100 ng





Epicypher熱銷產(chǎn)品——CUTANA™ pAG-Tn5 for CUT&Tag

如需了解更多詳細信息或相關產(chǎn)品，請聯(lián)系EpiCypher中國授權代理商-欣博盛生物