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Enzo Life Sciences的ROS-ID® NO Detection kit以紅色熒光的NO檢測(cè)試劑為主要成分,可通過熒光顯微鏡直接實(shí)時(shí)監(jiān)測(cè)活細(xì)胞中NO的產(chǎn)生。這種非熒光的、可滲透細(xì)胞的NO檢測(cè)染料在O2存在的情況下能夠與NO發(fā)生反應(yīng),具有高特異性、靈敏性和準(zhǔn)確性,產(chǎn)生不溶于水的紅色熒光產(chǎn)物。這種染料對(duì)過氧亞硝酸鹽沒有反應(yīng)性,因此可以區(qū)分過氧亞硝酸鹽和一氧化氮。
ROS-ID® NO Detection kit中的熒光探針對(duì)活性氯或溴不敏感,因此不用于檢測(cè)這些分析物。染色后,該染料產(chǎn)生的熒光產(chǎn)物可通過熒光顯微鏡進(jìn)行觀察。當(dāng)需要同時(shí)檢測(cè)額外的熒光信號(hào)(綠色或橙色)時(shí),建議使用650/670 nm的組合。ROS-ID® NO Detection kit包含足夠的試劑,可對(duì)活細(xì)胞(貼壁或懸浮)進(jìn)行至少200次檢測(cè)。
產(chǎn)品特色
通過熒光顯微鏡直接實(shí)時(shí)監(jiān)測(cè)活體細(xì)胞中NO的產(chǎn)生
高特異性、靈敏性和準(zhǔn)確性,產(chǎn)生不溶于水的紅色熒光產(chǎn)物
可區(qū)分過氧亞硝酸鹽和一氧化氮
試劑盒內(nèi)包含一氧化氮誘導(dǎo)劑和清除劑
產(chǎn)品信息
產(chǎn)品貨號(hào) | ENZ-51013-200 |
產(chǎn)品名稱 | ROS-ID® NO Detection kit / 欣博盛生物 |
規(guī)格 | 1*1Kit |
操作 | Protect from light. Avoid freeze/thaw cycles. |
短期保存 | -20°C |
長(zhǎng)期保存 | -80°C |
試劑盒組分 | NO Detection Reagent (Red), 60 µl NO Inducer (L-Arginine), 100 µl NO Scavenger (c-PTIO), 400 nmoles 10X Wash Buffer, 15 ml |
應(yīng)用 | Fluorescence microscopy, Fluorescent detection |
部分產(chǎn)品引用文獻(xiàn)
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